Anal Bioanal Chem 390:541ĭe Bièvre P, Barnes I (1985) Table of the isotopic composition of the elements as determined by mass-spectrometry. Sturup S, Hansen HR, Gammelgaard B (2008) Application of enriched stable isotopes as tracers in biological systems: a critical review. Cancer Res 61:5065įranklin P, Pullen R, Hall G (1992) Blood–brain exchange routes and distribution of Zn-65 in rat-brain. Takeda A, Tamano H, Enomoto S et al (2001) Zinc-65 imaging of rat brain tumors. Page V, Weisskopf L, Feller U (2006) Heavy metals in white lupin: uptake, root-to-shoot transfer and redistribution within the plant. Vallee B, Auld D (1993) Zinc-biological functions and coordination motifs. Using the bioimages and pool measurements from one physiological feature, it was possible to show that the aorta cell walls incorporate the zinc tracer at the different time points. When different time points have to be measured, higher precisions are required and therefore a larger area needs to be ablated (1 mm 2). With the parameters used, features in the tissue thin sections of at least 250 μm 2 in size are necessary to detect the incorporation of a tracer. It was established that at least 50 measurements from the microsampling were necessary to distinguish between controls and a tracer treated rat resulting in reduced resolution of the bioimage. Accumulated tracers which made up more than 0.1% of total zinc could be identified in the tissues of the treated rats. The isotope ratios determined by laser ablation ICP-MS were evaluated by additional measurements of tissue digests. A double tracer ( 70Zn and 67Zn) experiment with rats was designed to label the exchangeable zinc pool as well as the stable zinc pool. Here, we study the feasibility to use laser ablation coupled to quadrupole ICP-MS for the determination of zinc tracers given to rats at different time points with the aim to generate isotope ratio bioimages of heart tissue. Since the compartments might belong to physiological subsections of different organs, there is a need for microsampling analysis to determine isotope ratios of the trace element zinc in tissue samples. Stable isotopes of zinc can be used to identify body zinc compartments that have different turnover kinetics. The kinetics of zinc absorption, metabolism and excretion is extensively studied by nutritionists.
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